Friday, March 30, 2012

Transgenerational actions of environmental compounds on reproductive disease and identification of epigenetic biomarkers of ancestral exposures.


Manikkam M, Guerrero-Bosagna C, Tracey R, Haque MM, Skinner MK.
Source
Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, Washington, United States of America.
Abstract
Environmental factors during fetal development can induce a permanent epigenetic change in the germ line (sperm) that then transmits epigenetic transgenerational inheritance of adult-onset disease in the absence of any subsequent exposure. The epigenetic transgenerational actions of various environmental compounds and relevant mixtures were investigated with the use of a pesticide mixture (permethrin and insect repellant DEET), a plastic mixture (bisphenol A and phthalates), dioxin (TCDD) and a hydrocarbon mixture (jet fuel, JP8). After transient exposure of F0 gestating female rats during the period of embryonic gonadal sex determination, the subsequent F1-F3 generations were obtained in the absence of any environmental exposure. The effects on the F1, F2 and F3 generations pubertal onset and gonadal function were assessed. The plastics, dioxin and jet fuel were found to promote early-onset female puberty transgenerationally (F3 generation). Spermatogenic cell apoptosis was affected transgenerationally. Ovarian primordial follicle pool size was significantly decreased with all treatments transgenerationally. Differential DNA methylation of the F3 generation sperm promoter epigenome was examined. Differential DNA methylation regions (DMR) were identified in the sperm of all exposure lineage males and found to be consistent within a specific exposure lineage, but different between the exposures. Several genomic features of the DMR, such as low density CpG content, were identified. Exposure-specific epigenetic biomarkers were identified that may allow for the assessment of ancestral environmental exposures associated with adult onset disease.

3 comments:

  1. Environmental factors during fetal development can induce a permanent epigenetic change in the germ line (sperm) that then transmits epigenetic transgenerational inheritance of adult-onset disease in the absence of any subsequent exposure.

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  2. Judith ParkhouseApril 4, 2012 at 7:35 PM

    James Parkhouse was born on Friday the 13th, August 1948, He served 2 tours in Vietnam, 67-69, the worst of it. And another tour in Korea. He died on April Fool's Day 2012 in our home. Cancer had eaten up all of his insides. Agent Orange killed him. I read the obituaries, and all of our Vietnam veterans die way too soon. He was 63 years old. He was too young to die.

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  3. Words can not discribe(sp) how bad I feel after reading your comments. I was on the Son-Ong-Doc in late '69 & '70. I have had a 4V CABG, nephrectomy, bladder ca, DM II & psych problems. James is with God and all the Saints, no longer to suffer VN maladies. I will dedicate my 15:00 hrs. Mass today in his name. Yes, vn vets are dying off a way too soon.

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